Evaluation of ELISA for detection of rabies antibodies in domestic carnivores.
Identifieur interne : 000261 ( Main/Exploration ); précédent : 000260; suivant : 000262Evaluation of ELISA for detection of rabies antibodies in domestic carnivores.
Auteurs : Marine Wasniewski [France] ; Florence CliquetSource :
- Journal of virological methods [ 1879-0984 ] ; 2012.
English descriptors
- KwdEn :
- MESH :
- chemical , blood : Antibodies, Neutralizing, Antibodies, Viral.
- immunology : Rabies virus.
- methods : Enzyme-Linked Immunosorbent Assay, Neutralization Tests, Serologic Tests.
- prevention & control : Rabies.
- veterinary : Rabies.
- Animals, Cats, Dogs, Sensitivity and Specificity.
Abstract
Serological tests of pets have increased as many rabies-free countries have amended their quarantine measures and adopted a scheme requiring rabies vaccination followed by a serological test. A European directive requires the measurement of neutralising antibodies as proof of protection to allow the free movement of pets within the European Union and between third countries non listed in the list C of regulation 998/2003 and European countries. At present, the recommended neutralisation tests (FAVN test or RFFIT) are time-consuming, expensive and require highly trained technicians as well as special laboratory facilities. The rabies ELISA designed by BioPro was developed initially for use for field samples from foxes to check the efficacy of oral vaccination campaigns in Europe. In this study, the specificity, sensitivity and reliability of this commercial rabies ELISA was evaluated for testing sera from dogs and cats involved in international trade. The specificity evaluated in 315 unvaccinated animals was 100%. Concordance of 86.2% was obtained when comparing BioPro ELISA to the gold standard FAVN test in 701 samples from vaccinated dogs and cats. The rabies ELISA developed recently can be considered a valuable method for the assessment of rabies antibodies in vaccinated domestic carnivores in combination with neutralisation tests.
DOI: 10.1016/j.jviromet.2011.10.019
PubMed: 22080853
Affiliations:
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Le document en format XML
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Rabies, European Union Reference Laboratory for Rabies Serology, Technopôle agricole et vétérinaire, BP 40009, 54220 Malzéville Cedex, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>ANSES, Nancy Laboratory for Rabies and Wildlife, WHO Collaborating Centre for Research and Management in Zoonoses Control, OIE Reference Laboratory for Rabies, European Union Reference Laboratory for Rabies, European Union Reference Laboratory for Rabies Serology, Technopôle agricole et vétérinaire, BP 40009, 54220 Malzéville Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Lorraine (région)</region><settlement type="city">Malzéville</settlement></placeName></affiliation></author><author><name sortKey="Cliquet, Florence" sort="Cliquet, Florence" uniqKey="Cliquet F" first="Florence" last="Cliquet">Florence Cliquet</name></author></analytic><series><title level="j">Journal of virological 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Assay</term><term>Neutralization Tests</term><term>Serologic Tests</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Rabies</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Rabies</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cats</term><term>Dogs</term><term>Sensitivity and Specificity</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Serological tests of pets have increased as many rabies-free countries have amended their quarantine measures and adopted a scheme requiring rabies vaccination followed by a serological test. A European directive requires the measurement of neutralising antibodies as proof of protection to allow the free movement of pets within the European Union and between third countries non listed in the list C of regulation 998/2003 and European countries. At present, the recommended neutralisation tests (FAVN test or RFFIT) are time-consuming, expensive and require highly trained technicians as well as special laboratory facilities. The rabies ELISA designed by BioPro was developed initially for use for field samples from foxes to check the efficacy of oral vaccination campaigns in Europe. In this study, the specificity, sensitivity and reliability of this commercial rabies ELISA was evaluated for testing sera from dogs and cats involved in international trade. The specificity evaluated in 315 unvaccinated animals was 100%. Concordance of 86.2% was obtained when comparing BioPro ELISA to the gold standard FAVN test in 701 samples from vaccinated dogs and cats. The rabies ELISA developed recently can be considered a valuable method for the assessment of rabies antibodies in vaccinated domestic carnivores in combination with neutralisation tests.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Grand Est</li><li>Lorraine (région)</li></region><settlement><li>Malzéville</li></settlement></list><tree><noCountry><name sortKey="Cliquet, Florence" sort="Cliquet, Florence" uniqKey="Cliquet F" first="Florence" last="Cliquet">Florence Cliquet</name></noCountry><country name="France"><region name="Grand Est"><name sortKey="Wasniewski, Marine" sort="Wasniewski, Marine" uniqKey="Wasniewski M" first="Marine" last="Wasniewski">Marine Wasniewski</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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